Larry Murdock

Professor, Insect Physiology, International Crop Protection
 
Office Phone: 494-4592
Office Number: WSLR 124
Lab Number: WSLR 117
Office Phone: 494-4593
Email Address: murdockl@purdue.edu


Education

BAChemistry 1964, Depauw U.
MSEntomology 1966, Kansas State U.
PhDEntomology 1969, Kansas State U.


Research Interests

(1)Physiological and biochemical systems in insects that are vulnerable to plant defense proteins, with especial focus on the use of new genomic tools.
(2)Application of molecular biology to bring new sources of insect resistance into crop plants.
(3)Genetic improvement of cowpea, particularly as regards insects that constrain cowpea production and utilization.
(4)International agriculture, especially in Africa, focusing on the development and dissemination of simple, low-cost technologies for the post-harvest preservation of cowpea grain.


Professional Activities

2000- Created, with Virginia Ferris and Jeff Stuart, MPRINT (Molecular Plant Resistance to Insects and Nematodes Team), a consortium of Purdue scientists centered in the Department of Entomology dedicated to understanding and exploiting plant resistance to insects and nematodes.


Research and Other Awards

2001 Kansas State University Distinguished Alumnus in Entomology


Courses Taught

ENTM 551Insect Physiology and Biochemistry


Selected Publications

Refereed:
Koiwa H, Shade RE, Zhu-Salzman K, Subramanian L, Murdock LL, Nielsen SS, Bressan RA, Hasegawa PM (1998) Phage display selection can differentiate insecticidal activity of soybean cystatins. Plant J. 14: 371-379.
Kitch LW, Boukar O, Endondo C, Murdock, LL (1998) Farmer acceptability criteria in breeding cowpea. Exp. Agr. 34: 475-486.
Zhu-Salzman K, Shade RE, Koiwa H, Salzman RA, Narasimhan M, Bressan RA, Hasegawa PM, Murdock LL (1998) Carbohydrate binding and resistance to proteolysis control insecticidal activity of Griffonia simplicifolia lectin II.
P. Natl Acad. Sci. USA 95: 15123-15128.
Zhu-Salzman K, Salzman RA, Koiwa H, Murdock LL, Bressan RA, Hasegawa PM (1998) Ethylene negatively regulates local expression of plant defense lectin genes. Physiol. Plantarum 104: 365-372.
Shade RE, Murdock LL, Kitch LW (2000) Interactions between cowpea weevil (Coleoptera : Bruchidae) populations and Vigna (Leguminosae) species. J Econ. Entomol. 92: 740-745.
Koiwa H, Shade RE, Zhu-Salzman K, D'Urzo MP, Murdock LL, Bressan RA, Hasegawa PM (2000) A plant defensive cystatin (soyacystatin) targets cathepsin L-like digestive cysteine proteinases (DvCALs) in the larval midgut of western corn rootworm (Diabrotica virgifera virgifera). FEBS Lett. 471: 67-70.
Pittendrigh BR, Gaffney P, Murdock LL (2000) Deterministic modeling of negative cross-resistance strategies for use in transgenic host-plant resistance. J Theoret. Biol. 204: 135-150
Koiwa H, D'Urzo MP, Zhu-Salzman K, Ibeas JI, Shade RE, Murdock LL, Bressan RA, Hasegawa PM (2000) An in-gel assay of a recombinant western corn rootworm (Diabrotica virgifera virgifera) cysteine proteinase expressed in yeast Anal. Biochem 282: 153-155.
Koiwa H, Subramanian L, Shade RE, Zhu KY, Nielsen SS, Murdock LL, Bressan RA, Hasegawa PM (1997) Insecticidal activities of soybean cysteine proteinase inhibitors are correlated with their papain-binding affinities as phage-displayed proteins (1997) Plant Physiol. 114: 1115-1115 Suppl.
Koiwa H, D'Urzo MP, Assfalg-Machleidt I, Zhu-Salzman K, Shade RE, An HJ, Murdock LL, Machleidt W, Bressan RA, Hasegawa PM (2001) Phage display selection of hairpin loop soyacystatin variants that mediate high affinity inhibition of a cysteine proteinase. Plant J. 27, 393-391.
Pittendrigh BR, Huesing JE, Shade RE, Murdock LL (1997) Monitoring of rice weevil, Sitophilus oryzae, feeding behavior in maize seeds and the occurrence of supernumerary molts in low humidity conditions. Entomol. Exp. Appl. 83: 225-231.
Shade RE, Schroeder HE, Pueyo JJ, Tabe LM, Murdock LL, Higgins TJV, Chrispeels MJ. (1994) Transgenic pea seeds expressing the alpha amylase inhibitor of the common bean are resistant to bruchid beetles. Bio/Technology 12: 793-796.
Zhu KY, Huesing JE, Shade RE, Murdock LL. (1994) Cowpea trypsin inhibitor and resistance to cowpea weevil (Coleoptera: Bruchidae) in cowpea variety Tvu 2027. Environ. Entomol. 23: 987-991.
Wolfson JL, Murdock LL (1995) Potential use of protease inhibitors for host plant resistance – a test case. Environ. Entomol. 24: 52-57.
Zhu KY, Huesing JE, Shade RE, Bressan RA, Hasegawa PM, Murdock LL. (1996)
An insecticidal N-acetylglucosamine-specific lectin gene from Griffonia simplicifolia (Leguminosae). Plant Physiol. 110: 195-202.
Zhu KY, Bressan RA, Hasegawa PM, Murdock LL (1996). Identification of N-acetylglucosamine binding residues in Griffonia simplicifolia lectin II. FEBS Lett. 390: 271-274.
Shade RE, Kitch LW, Mentzer P, Murdock LL (1996) Selection of a cowpea weevil (Coleoptera: Bruchidae) biotype virulent to cowpea weevil resistant landrace TVu 2027. J. Econ. Entomol. 89: 1325-1331.
Pittendrigh BR, Huesing JE, Shade RE, Murdock LL (1997). Effects of lectins, CRY1A/CRY1B Bt delta-endotoxin, PAPA, protease and alpha-amylase inhibitors, on the development of the rice weevil, Sitophilus oryzae, using an artificial seed bioassay. Entomol. Exp. Appl. 82: 201-211.
Ntoukam G, Kitch LW, Shade RE, Murdock LL (1997) A novel method for conserving cowpea germplasm and breeding stocks using solar disinfestations. J Stored Prod. Res. 33: 175-179.
Murdock LL, and Shade RE (2002) Lectins and protease inhibitors as plant defenses against insects. Ag Food Chem (special issue, in press).
Book Chapters/Proceedings:
L.L. Murdock (2001) The Dakar Symposium/Workshop on the Genetic Improvement of Cowpea. Proceedings Report.
A.B. Salifu and L.L. Murdock (in preparation) Integrated Pest Management in Cowpea.
L.L. Murdock, Shade R.E., Kitch L.W., Ntoukam, G. Lowenberg-Deboer, J. Huesing, J.E., Moar, W., Chambliss, O.L., Endondo, C., and Wolfson, J.L. (1997). Postharvest storage of cowpea in sub-Saharan Africa. In B. B. Singh et al. [eds.] Advances in Cowpea Research. Copublication of IITA and JIRCAS. IITA, Ibadan, Nigeria, pp. 302-312.
L.M. Monti, L.L. Murdock, and G. Thottappilly (1997) Opportunities for Biotechnology in Cowpea. In B. B. Singh et al. [eds.] Advances in Cowpea Research. Copublication of IITA and JIRCAS. IITA, Ibadan, Nigeria, pp. 302-312.
L.L. Murdock (1999) Agricultural science (plant). McGraw-Hill Yearbook of Science and Technology, pp. 11-12.
Kononowicz, A.K., Cheah, M.L., Narasimhan, M.L., Murdock, L.L., Shade, R.E., Chrispeels, M.J., Filippone, E., Monti, L.M., Bressan, R.A., and Hasegawa, P.M. (1997). Developing a transformation system for cowpea (Vigna unguiculata [L] Walp.). In B. B. Singh et al. [eds.] Advances in Cowpea Research. Copublication of IITA and JIRCAS. IITA, Ibadan, Nigeria, pp. 302-312.
Patents (issued and/or pending):
B.R. Pittendrigh, P. Gaffney and L.L. Murdock. Method for Screening Negative Cross Resistance. PCT Patent Application No. PCT/US01/18062.